Analysis of stimulation experiments.

library(Seurat)
library(dplyr)
library(ggplot2)
library(scClustViz)
library(SeuratWrappers)

Load integrated object. This object has been integrated with CCA, because there was lots of batch effect between the 0hr tissue and the other two experiments (which merged quite well).

load("~/Dropbox/Zoe/scf_version/analysis/healthy_sc/seurat_objects/no_dropletQC/3391/integrated_3391_stimulation_pmaio_unstim_downsampled_cca_kanchor5_scClustViz.RData")
# Set identity
Idents(scSeurat) <- "integrated_snn_res.0.4"

Check out the UMAP of clusters, original identities, and refined SCINA labels.

DimPlot(scSeurat, label = TRUE)

DimPlot(scSeurat, group.by = "tissue_type", label = FALSE)

DimPlot(scSeurat, group.by = "scina_labels", label = TRUE) & NoLegend()

pdf(file = './figures/stimulation/downsampled_UMAP.pdf')
DimPlot(scSeurat, label = TRUE)
dev.off()
null device 
          1 
pdf(file = './figures/stimulation/downsampled_tissue_type_UMAP.pdf')
DimPlot(scSeurat, group.by = "tissue_type") +
  ggtitle(NULL)
dev.off()
null device 
          1 

Create UMAP of general cell-type labels

plot <- DimPlot(scSeurat, group.by = "general_cell_labels_clusts", label = TRUE, repel = TRUE) +
  labs(title = NULL) & NoLegend()
plot
pdf("./figures/stimulation/downsampled_general_cell_type_UMAP.pdf",
    height = 5, width = 7)
plot
dev.off()
png 
  2 

Look at tissue-type per cluster on a proportional level:

plot + coord_flip()
pdf(file = './figures/stimulation/downsampled_tissue_type_barplot.pdf',
    height = 5, width = 7)
plot + coord_flip()
dev.off()
png 
  2 

Calculate all DE genes

all.markers <- FindAllMarkers(scSeurat,
                              only.pos = TRUE,
                              min.pct = 0.25, 
                              logfc.threshold = 0.25,
                              recorrect_umi = FALSE)
Calculating cluster 0

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Calculating cluster 1

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Calculating cluster 2

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Calculating cluster 3

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Calculating cluster 4

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Calculating cluster 5

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  |++++++++++                                        | 20% ~08s          
  |+++++++++++                                       | 21% ~08s          
  |+++++++++++                                       | 22% ~08s          
  |++++++++++++                                      | 23% ~08s          
  |+++++++++++++                                     | 24% ~07s          
  |+++++++++++++                                     | 25% ~07s          
  |++++++++++++++                                    | 26% ~07s          
  |++++++++++++++                                    | 27% ~07s          
  |+++++++++++++++                                   | 29% ~07s          
  |+++++++++++++++                                   | 30% ~07s          
  |++++++++++++++++                                  | 31% ~07s          
  |++++++++++++++++                                  | 32% ~07s          
  |+++++++++++++++++                                 | 33% ~07s          
  |++++++++++++++++++                                | 34% ~06s          
  |++++++++++++++++++                                | 35% ~06s          
  |+++++++++++++++++++                               | 36% ~06s          
  |+++++++++++++++++++                               | 37% ~06s          
  |++++++++++++++++++++                              | 38% ~06s          
  |++++++++++++++++++++                              | 40% ~06s          
  |+++++++++++++++++++++                             | 41% ~06s          
  |+++++++++++++++++++++                             | 42% ~06s          
  |++++++++++++++++++++++                            | 43% ~06s          
  |++++++++++++++++++++++                            | 44% ~06s          
  |+++++++++++++++++++++++                           | 45% ~05s          
  |++++++++++++++++++++++++                          | 46% ~05s          
  |++++++++++++++++++++++++                          | 47% ~05s          
  |+++++++++++++++++++++++++                         | 48% ~05s          
  |+++++++++++++++++++++++++                         | 49% ~05s          
  |++++++++++++++++++++++++++                        | 51% ~05s          
  |++++++++++++++++++++++++++                        | 52% ~05s          
  |+++++++++++++++++++++++++++                       | 53% ~05s          
  |+++++++++++++++++++++++++++                       | 54% ~05s          
  |++++++++++++++++++++++++++++                      | 55% ~04s          
  |+++++++++++++++++++++++++++++                     | 56% ~04s          
  |+++++++++++++++++++++++++++++                     | 57% ~04s          
  |++++++++++++++++++++++++++++++                    | 58% ~04s          
  |++++++++++++++++++++++++++++++                    | 59% ~04s          
  |+++++++++++++++++++++++++++++++                   | 60% ~04s          
  |+++++++++++++++++++++++++++++++                   | 62% ~04s          
  |++++++++++++++++++++++++++++++++                  | 63% ~04s          
  |++++++++++++++++++++++++++++++++                  | 64% ~04s          
  |+++++++++++++++++++++++++++++++++                 | 65% ~03s          
  |+++++++++++++++++++++++++++++++++                 | 66% ~03s          
  |++++++++++++++++++++++++++++++++++                | 67% ~03s          
  |+++++++++++++++++++++++++++++++++++               | 68% ~03s          
  |+++++++++++++++++++++++++++++++++++               | 69% ~03s          
  |++++++++++++++++++++++++++++++++++++              | 70% ~03s          
  |++++++++++++++++++++++++++++++++++++              | 71% ~03s          
  |+++++++++++++++++++++++++++++++++++++             | 73% ~03s          
  |+++++++++++++++++++++++++++++++++++++             | 74% ~03s          
  |++++++++++++++++++++++++++++++++++++++            | 75% ~03s          
  |++++++++++++++++++++++++++++++++++++++            | 76% ~02s          
  |+++++++++++++++++++++++++++++++++++++++           | 77% ~02s          
  |++++++++++++++++++++++++++++++++++++++++          | 78% ~02s          
  |++++++++++++++++++++++++++++++++++++++++          | 79% ~02s          
  |+++++++++++++++++++++++++++++++++++++++++         | 80% ~02s          
  |+++++++++++++++++++++++++++++++++++++++++         | 81% ~02s          
  |++++++++++++++++++++++++++++++++++++++++++        | 82% ~02s          
  |++++++++++++++++++++++++++++++++++++++++++        | 84% ~02s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~02s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 86% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 88% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 90% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 92% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~00s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~00s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=10s  
Calculating cluster 6

  |                                                  | 0 % ~calculating  
  |+                                                 | 1 % ~07s          
  |++                                                | 2 % ~07s          
  |++                                                | 4 % ~06s          
  |+++                                               | 5 % ~06s          
  |+++                                               | 6 % ~06s          
  |++++                                              | 7 % ~06s          
  |+++++                                             | 8 % ~06s          
  |+++++                                             | 9 % ~06s          
  |++++++                                            | 11% ~06s          
  |++++++                                            | 12% ~06s          
  |+++++++                                           | 13% ~06s          
  |++++++++                                          | 14% ~06s          
  |++++++++                                          | 15% ~06s          
  |+++++++++                                         | 16% ~05s          
  |+++++++++                                         | 18% ~05s          
  |++++++++++                                        | 19% ~05s          
  |++++++++++                                        | 20% ~05s          
  |+++++++++++                                       | 21% ~05s          
  |++++++++++++                                      | 22% ~05s          
  |++++++++++++                                      | 24% ~05s          
  |+++++++++++++                                     | 25% ~05s          
  |+++++++++++++                                     | 26% ~05s          
  |++++++++++++++                                    | 27% ~05s          
  |+++++++++++++++                                   | 28% ~05s          
  |+++++++++++++++                                   | 29% ~05s          
  |++++++++++++++++                                  | 31% ~05s          
  |++++++++++++++++                                  | 32% ~05s          
  |+++++++++++++++++                                 | 33% ~04s          
  |++++++++++++++++++                                | 34% ~04s          
  |++++++++++++++++++                                | 35% ~04s          
  |+++++++++++++++++++                               | 36% ~04s          
  |+++++++++++++++++++                               | 38% ~04s          
  |++++++++++++++++++++                              | 39% ~04s          
  |++++++++++++++++++++                              | 40% ~04s          
  |+++++++++++++++++++++                             | 41% ~04s          
  |++++++++++++++++++++++                            | 42% ~04s          
  |++++++++++++++++++++++                            | 44% ~04s          
  |+++++++++++++++++++++++                           | 45% ~04s          
  |+++++++++++++++++++++++                           | 46% ~04s          
  |++++++++++++++++++++++++                          | 47% ~04s          
  |+++++++++++++++++++++++++                         | 48% ~03s          
  |+++++++++++++++++++++++++                         | 49% ~03s          
  |++++++++++++++++++++++++++                        | 51% ~03s          
  |++++++++++++++++++++++++++                        | 52% ~03s          
  |+++++++++++++++++++++++++++                       | 53% ~03s          
  |++++++++++++++++++++++++++++                      | 54% ~03s          
  |++++++++++++++++++++++++++++                      | 55% ~03s          
  |+++++++++++++++++++++++++++++                     | 56% ~03s          
  |+++++++++++++++++++++++++++++                     | 58% ~03s          
  |++++++++++++++++++++++++++++++                    | 59% ~03s          
  |++++++++++++++++++++++++++++++                    | 60% ~03s          
  |+++++++++++++++++++++++++++++++                   | 61% ~03s          
  |++++++++++++++++++++++++++++++++                  | 62% ~02s          
  |++++++++++++++++++++++++++++++++                  | 64% ~02s          
  |+++++++++++++++++++++++++++++++++                 | 65% ~02s          
  |+++++++++++++++++++++++++++++++++                 | 66% ~02s          
  |++++++++++++++++++++++++++++++++++                | 67% ~02s          
  |+++++++++++++++++++++++++++++++++++               | 68% ~02s          
  |+++++++++++++++++++++++++++++++++++               | 69% ~02s          
  |++++++++++++++++++++++++++++++++++++              | 71% ~02s          
  |++++++++++++++++++++++++++++++++++++              | 72% ~02s          
  |+++++++++++++++++++++++++++++++++++++             | 73% ~02s          
  |++++++++++++++++++++++++++++++++++++++            | 74% ~02s          
  |++++++++++++++++++++++++++++++++++++++            | 75% ~02s          
  |+++++++++++++++++++++++++++++++++++++++           | 76% ~02s          
  |+++++++++++++++++++++++++++++++++++++++           | 78% ~01s          
  |++++++++++++++++++++++++++++++++++++++++          | 79% ~01s          
  |++++++++++++++++++++++++++++++++++++++++          | 80% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++         | 81% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++        | 82% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++        | 84% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 86% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 88% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 94% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~00s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 96% ~00s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=07s  
Calculating cluster 7

  |                                                  | 0 % ~calculating  
  |+                                                 | 1 % ~05s          
  |++                                                | 2 % ~05s          
  |++                                                | 4 % ~05s          
  |+++                                               | 5 % ~05s          
  |++++                                              | 6 % ~05s          
  |++++                                              | 7 % ~05s          
  |+++++                                             | 9 % ~05s          
  |+++++                                             | 10% ~05s          
  |++++++                                            | 11% ~05s          
  |+++++++                                           | 12% ~05s          
  |+++++++                                           | 13% ~05s          
  |++++++++                                          | 15% ~05s          
  |++++++++                                          | 16% ~04s          
  |+++++++++                                         | 17% ~04s          
  |++++++++++                                        | 18% ~04s          
  |++++++++++                                        | 20% ~04s          
  |+++++++++++                                       | 21% ~04s          
  |+++++++++++                                       | 22% ~04s          
  |++++++++++++                                      | 23% ~04s          
  |+++++++++++++                                     | 24% ~04s          
  |+++++++++++++                                     | 26% ~04s          
  |++++++++++++++                                    | 27% ~04s          
  |+++++++++++++++                                   | 28% ~04s          
  |+++++++++++++++                                   | 29% ~04s          
  |++++++++++++++++                                  | 30% ~04s          
  |++++++++++++++++                                  | 32% ~04s          
  |+++++++++++++++++                                 | 33% ~04s          
  |++++++++++++++++++                                | 34% ~04s          
  |++++++++++++++++++                                | 35% ~04s          
  |+++++++++++++++++++                               | 37% ~03s          
  |+++++++++++++++++++                               | 38% ~03s          
  |++++++++++++++++++++                              | 39% ~03s          
  |+++++++++++++++++++++                             | 40% ~03s          
  |+++++++++++++++++++++                             | 41% ~03s          
  |++++++++++++++++++++++                            | 43% ~03s          
  |++++++++++++++++++++++                            | 44% ~03s          
  |+++++++++++++++++++++++                           | 45% ~03s          
  |++++++++++++++++++++++++                          | 46% ~03s          
  |++++++++++++++++++++++++                          | 48% ~03s          
  |+++++++++++++++++++++++++                         | 49% ~03s          
  |+++++++++++++++++++++++++                         | 50% ~03s          
  |++++++++++++++++++++++++++                        | 51% ~03s          
  |+++++++++++++++++++++++++++                       | 52% ~03s          
  |+++++++++++++++++++++++++++                       | 54% ~03s          
  |++++++++++++++++++++++++++++                      | 55% ~02s          
  |+++++++++++++++++++++++++++++                     | 56% ~02s          
  |+++++++++++++++++++++++++++++                     | 57% ~02s          
  |++++++++++++++++++++++++++++++                    | 59% ~02s          
  |++++++++++++++++++++++++++++++                    | 60% ~02s          
  |+++++++++++++++++++++++++++++++                   | 61% ~02s          
  |++++++++++++++++++++++++++++++++                  | 62% ~02s          
  |++++++++++++++++++++++++++++++++                  | 63% ~02s          
  |+++++++++++++++++++++++++++++++++                 | 65% ~02s          
  |+++++++++++++++++++++++++++++++++                 | 66% ~02s          
  |++++++++++++++++++++++++++++++++++                | 67% ~02s          
  |+++++++++++++++++++++++++++++++++++               | 68% ~02s          
  |+++++++++++++++++++++++++++++++++++               | 70% ~02s          
  |++++++++++++++++++++++++++++++++++++              | 71% ~02s          
  |++++++++++++++++++++++++++++++++++++              | 72% ~02s          
  |+++++++++++++++++++++++++++++++++++++             | 73% ~01s          
  |++++++++++++++++++++++++++++++++++++++            | 74% ~01s          
  |++++++++++++++++++++++++++++++++++++++            | 76% ~01s          
  |+++++++++++++++++++++++++++++++++++++++           | 77% ~01s          
  |++++++++++++++++++++++++++++++++++++++++          | 78% ~01s          
  |++++++++++++++++++++++++++++++++++++++++          | 79% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++         | 80% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++         | 82% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++        | 83% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 84% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 88% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 90% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~00s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~00s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~00s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 96% ~00s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=05s  
Calculating cluster 8

  |                                                  | 0 % ~calculating  
  |+                                                 | 1 % ~04s          
  |++                                                | 2 % ~04s          
  |++                                                | 3 % ~04s          
  |+++                                               | 4 % ~04s          
  |+++                                               | 5 % ~04s          
  |++++                                              | 6 % ~04s          
  |++++                                              | 7 % ~04s          
  |+++++                                             | 8 % ~04s          
  |+++++                                             | 9 % ~04s          
  |++++++                                            | 10% ~04s          
  |++++++                                            | 11% ~04s          
  |+++++++                                           | 12% ~04s          
  |+++++++                                           | 13% ~04s          
  |++++++++                                          | 14% ~04s          
  |++++++++                                          | 15% ~04s          
  |+++++++++                                         | 16% ~04s          
  |+++++++++                                         | 17% ~03s          
  |++++++++++                                        | 18% ~03s          
  |++++++++++                                        | 19% ~03s          
  |+++++++++++                                       | 20% ~03s          
  |+++++++++++                                       | 21% ~03s          
  |++++++++++++                                      | 22% ~03s          
  |++++++++++++                                      | 23% ~03s          
  |+++++++++++++                                     | 24% ~03s          
  |+++++++++++++                                     | 26% ~03s          
  |++++++++++++++                                    | 27% ~03s          
  |++++++++++++++                                    | 28% ~03s          
  |+++++++++++++++                                   | 29% ~03s          
  |+++++++++++++++                                   | 30% ~03s          
  |++++++++++++++++                                  | 31% ~03s          
  |++++++++++++++++                                  | 32% ~03s          
  |+++++++++++++++++                                 | 33% ~03s          
  |+++++++++++++++++                                 | 34% ~03s          
  |++++++++++++++++++                                | 35% ~03s          
  |++++++++++++++++++                                | 36% ~03s          
  |+++++++++++++++++++                               | 37% ~03s          
  |+++++++++++++++++++                               | 38% ~03s          
  |++++++++++++++++++++                              | 39% ~02s          
  |++++++++++++++++++++                              | 40% ~02s          
  |+++++++++++++++++++++                             | 41% ~02s          
  |+++++++++++++++++++++                             | 42% ~02s          
  |++++++++++++++++++++++                            | 43% ~02s          
  |++++++++++++++++++++++                            | 44% ~02s          
  |+++++++++++++++++++++++                           | 45% ~02s          
  |+++++++++++++++++++++++                           | 46% ~02s          
  |++++++++++++++++++++++++                          | 47% ~02s          
  |++++++++++++++++++++++++                          | 48% ~02s          
  |+++++++++++++++++++++++++                         | 49% ~02s          
  |+++++++++++++++++++++++++                         | 50% ~02s          
  |++++++++++++++++++++++++++                        | 51% ~02s          
  |+++++++++++++++++++++++++++                       | 52% ~02s          
  |+++++++++++++++++++++++++++                       | 53% ~02s          
  |++++++++++++++++++++++++++++                      | 54% ~02s          
  |++++++++++++++++++++++++++++                      | 55% ~02s          
  |+++++++++++++++++++++++++++++                     | 56% ~02s          
  |+++++++++++++++++++++++++++++                     | 57% ~02s          
  |++++++++++++++++++++++++++++++                    | 58% ~02s          
  |++++++++++++++++++++++++++++++                    | 59% ~02s          
  |+++++++++++++++++++++++++++++++                   | 60% ~02s          
  |+++++++++++++++++++++++++++++++                   | 61% ~02s          
  |++++++++++++++++++++++++++++++++                  | 62% ~02s          
  |++++++++++++++++++++++++++++++++                  | 63% ~01s          
  |+++++++++++++++++++++++++++++++++                 | 64% ~01s          
  |+++++++++++++++++++++++++++++++++                 | 65% ~01s          
  |++++++++++++++++++++++++++++++++++                | 66% ~01s          
  |++++++++++++++++++++++++++++++++++                | 67% ~01s          
  |+++++++++++++++++++++++++++++++++++               | 68% ~01s          
  |+++++++++++++++++++++++++++++++++++               | 69% ~01s          
  |++++++++++++++++++++++++++++++++++++              | 70% ~01s          
  |++++++++++++++++++++++++++++++++++++              | 71% ~01s          
  |+++++++++++++++++++++++++++++++++++++             | 72% ~01s          
  |+++++++++++++++++++++++++++++++++++++             | 73% ~01s          
  |++++++++++++++++++++++++++++++++++++++            | 74% ~01s          
  |++++++++++++++++++++++++++++++++++++++            | 76% ~01s          
  |+++++++++++++++++++++++++++++++++++++++           | 77% ~01s          
  |+++++++++++++++++++++++++++++++++++++++           | 78% ~01s          
  |++++++++++++++++++++++++++++++++++++++++          | 79% ~01s          
  |++++++++++++++++++++++++++++++++++++++++          | 80% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++         | 81% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++         | 82% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++        | 83% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++        | 84% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 86% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 88% ~00s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~00s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 90% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~00s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~00s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 94% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 96% ~00s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~00s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=04s  
Calculating cluster 9

  |                                                  | 0 % ~calculating  
  |+                                                 | 1 % ~05s          
  |++                                                | 2 % ~05s          
  |++                                                | 3 % ~05s          
  |+++                                               | 5 % ~05s          
  |+++                                               | 6 % ~05s          
  |++++                                              | 7 % ~05s          
  |+++++                                             | 8 % ~05s          
  |+++++                                             | 9 % ~05s          
  |++++++                                            | 10% ~05s          
  |++++++                                            | 11% ~05s          
  |+++++++                                           | 13% ~05s          
  |+++++++                                           | 14% ~04s          
  |++++++++                                          | 15% ~04s          
  |+++++++++                                         | 16% ~04s          
  |+++++++++                                         | 17% ~04s          
  |++++++++++                                        | 18% ~04s          
  |++++++++++                                        | 20% ~04s          
  |+++++++++++                                       | 21% ~04s          
  |+++++++++++                                       | 22% ~04s          
  |++++++++++++                                      | 23% ~04s          
  |+++++++++++++                                     | 24% ~04s          
  |+++++++++++++                                     | 25% ~04s          
  |++++++++++++++                                    | 26% ~04s          
  |++++++++++++++                                    | 28% ~04s          
  |+++++++++++++++                                   | 29% ~04s          
  |+++++++++++++++                                   | 30% ~04s          
  |++++++++++++++++                                  | 31% ~04s          
  |+++++++++++++++++                                 | 32% ~04s          
  |+++++++++++++++++                                 | 33% ~03s          
  |++++++++++++++++++                                | 34% ~03s          
  |++++++++++++++++++                                | 36% ~03s          
  |+++++++++++++++++++                               | 37% ~03s          
  |+++++++++++++++++++                               | 38% ~03s          
  |++++++++++++++++++++                              | 39% ~03s          
  |+++++++++++++++++++++                             | 40% ~03s          
  |+++++++++++++++++++++                             | 41% ~03s          
  |++++++++++++++++++++++                            | 43% ~03s          
  |++++++++++++++++++++++                            | 44% ~03s          
  |+++++++++++++++++++++++                           | 45% ~03s          
  |+++++++++++++++++++++++                           | 46% ~03s          
  |++++++++++++++++++++++++                          | 47% ~03s          
  |+++++++++++++++++++++++++                         | 48% ~03s          
  |+++++++++++++++++++++++++                         | 49% ~03s          
  |++++++++++++++++++++++++++                        | 51% ~03s          
  |++++++++++++++++++++++++++                        | 52% ~03s          
  |+++++++++++++++++++++++++++                       | 53% ~02s          
  |++++++++++++++++++++++++++++                      | 54% ~02s          
  |++++++++++++++++++++++++++++                      | 55% ~02s          
  |+++++++++++++++++++++++++++++                     | 56% ~02s          
  |+++++++++++++++++++++++++++++                     | 57% ~02s          
  |++++++++++++++++++++++++++++++                    | 59% ~02s          
  |++++++++++++++++++++++++++++++                    | 60% ~02s          
  |+++++++++++++++++++++++++++++++                   | 61% ~02s          
  |++++++++++++++++++++++++++++++++                  | 62% ~02s          
  |++++++++++++++++++++++++++++++++                  | 63% ~02s          
  |+++++++++++++++++++++++++++++++++                 | 64% ~02s          
  |+++++++++++++++++++++++++++++++++                 | 66% ~02s          
  |++++++++++++++++++++++++++++++++++                | 67% ~02s          
  |++++++++++++++++++++++++++++++++++                | 68% ~02s          
  |+++++++++++++++++++++++++++++++++++               | 69% ~02s          
  |++++++++++++++++++++++++++++++++++++              | 70% ~02s          
  |++++++++++++++++++++++++++++++++++++              | 71% ~02s          
  |+++++++++++++++++++++++++++++++++++++             | 72% ~01s          
  |+++++++++++++++++++++++++++++++++++++             | 74% ~01s          
  |++++++++++++++++++++++++++++++++++++++            | 75% ~01s          
  |++++++++++++++++++++++++++++++++++++++            | 76% ~01s          
  |+++++++++++++++++++++++++++++++++++++++           | 77% ~01s          
  |++++++++++++++++++++++++++++++++++++++++          | 78% ~01s          
  |++++++++++++++++++++++++++++++++++++++++          | 79% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++         | 80% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++         | 82% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++        | 83% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++        | 84% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++       | 85% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 86% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++      | 87% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 89% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++++++     | 90% ~01s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 91% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++    | 92% ~00s          
  |+++++++++++++++++++++++++++++++++++++++++++++++   | 93% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 94% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++++  | 95% ~00s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~00s          
  |+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s          
  |++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=05s  
Calculating cluster 10

  |                                                  | 0 % ~calculating  
  |+                                                 | 1 % ~03s          
  |++                                                | 3 % ~03s          
  |+++                                               | 4 % ~02s          
  |+++                                               | 6 % ~02s          
  |++++                                              | 7 % ~02s          
  |+++++                                             | 8 % ~02s          
  |+++++                                             | 10% ~02s          
  |++++++                                            | 11% ~02s          
  |+++++++                                           | 13% ~02s          
  |++++++++                                          | 14% ~02s          
  |++++++++                                          | 15% ~02s          
  |+++++++++                                         | 17% ~02s          
  |++++++++++                                        | 18% ~02s          
  |++++++++++                                        | 20% ~02s          
  |+++++++++++                                       | 21% ~02s          
  |++++++++++++                                      | 23% ~02s          
  |++++++++++++                                      | 24% ~02s          
  |+++++++++++++                                     | 25% ~02s          
  |++++++++++++++                                    | 27% ~02s          
  |+++++++++++++++                                   | 28% ~02s          
  |+++++++++++++++                                   | 30% ~02s          
  |++++++++++++++++                                  | 31% ~02s          
  |+++++++++++++++++                                 | 32% ~02s          
  |+++++++++++++++++                                 | 34% ~02s          
  |++++++++++++++++++                                | 35% ~02s          
  |+++++++++++++++++++                               | 37% ~02s          
  |++++++++++++++++++++                              | 38% ~02s          
  |++++++++++++++++++++                              | 39% ~02s          
  |+++++++++++++++++++++                             | 41% ~02s          
  |++++++++++++++++++++++                            | 42% ~01s          
  |++++++++++++++++++++++                            | 44% ~01s          
  |+++++++++++++++++++++++                           | 45% ~01s          
  |++++++++++++++++++++++++                          | 46% ~01s          
  |++++++++++++++++++++++++                          | 48% ~01s          
  |+++++++++++++++++++++++++                         | 49% ~01s          
  |++++++++++++++++++++++++++                        | 51% ~01s          
  |+++++++++++++++++++++++++++                       | 52% ~01s          
  |+++++++++++++++++++++++++++                       | 54% ~01s          
  |++++++++++++++++++++++++++++                      | 55% ~01s          
  |+++++++++++++++++++++++++++++                     | 56% ~01s          
  |+++++++++++++++++++++++++++++                     | 58% ~01s          
  |++++++++++++++++++++++++++++++                    | 59% ~01s          
  |+++++++++++++++++++++++++++++++                   | 61% ~01s          
  |+++++++++++++++++++++++++++++++                   | 62% ~01s          
  |++++++++++++++++++++++++++++++++                  | 63% ~01s          
  |+++++++++++++++++++++++++++++++++                 | 65% ~01s          
  |++++++++++++++++++++++++++++++++++                | 66% ~01s          
  |++++++++++++++++++++++++++++++++++                | 68% ~01s          
  |+++++++++++++++++++++++++++++++++++               | 69% ~01s          
  |++++++++++++++++++++++++++++++++++++              | 70% ~01s          
  |++++++++++++++++++++++++++++++++++++              | 72% ~01s          
  |+++++++++++++++++++++++++++++++++++++             | 73% ~01s          
  |++++++++++++++++++++++++++++++++++++++            | 75% ~01s          
  |+++++++++++++++++++++++++++++++++++++++           | 76% ~01s          
  |+++++++++++++++++++++++++++++++++++++++           | 77% ~01s          
  |++++++++++++++++++++++++++++++++++++++++          | 79% ~01s          
  |+++++++++++++++++++++++++++++++++++++++++         | 80% ~01s          
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Calculating cluster 11

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Calculating cluster 12

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Calculating cluster 13

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top.markers <- all.markers %>% group_by(cluster) %>% top_n(n = 10, wt = avg_log2FC)
top.markers
write.table(all.markers,
            file = "./figures/stimulation/downsampled_cluster_markers.tsv",
            sep = "\t",
            quote = FALSE,
            row.names = FALSE)

Calculate DE genes: for each cluster, compare stim VS unstim

for (clust in levels(as.factor(Idents(scSeurat)))) {
  cells_stim <- colnames(scSeurat)[Idents(scSeurat) == clust & scSeurat$tissue_type == "6hrs_PMAIO"]
  cells_unstim <- colnames(scSeurat)[Idents(scSeurat) == clust & scSeurat$tissue_type == "6hrs_unstim"]
  markers <- RunPresto(scSeurat,
                       ident.1 = cells_stim,
                       ident.2 = cells_unstim,
                       only.pos = FALSE,
                       min.pct = 0.25,
                       logfc.threshold = 0.25,
                       recorrect_umi = FALSE)
  write.table(markers,
              file = paste("./figures/stimulation/downsampled_markers_cluster", 
                           clust ,"_stim_vs_unstim.tsv", sep = ""),
              sep = "\t",
              quote = FALSE,
              row.names = TRUE)
}

Look for T-cell signatures.

FeaturePlot(scSeurat, features = c("CD3E", "NKG7"))

FeaturePlot(scSeurat, features = c("LEF1", "XCL1;XCL2"))

FeaturePlot(scSeurat, features = c("TOX", "CD8A"))

FeaturePlot(scSeurat, features = c("CCR7"))

Look for macrophage signatures:

FeaturePlot(scSeurat, features = c("LGALS1", "TYROBP"))

FeaturePlot(scSeurat, features = c("MARCO", "C1QC"))

FeaturePlot(scSeurat, features = c("LYZ-1", "S100A8"))

FeaturePlot(scSeurat, features = c("DEFA6;DEFA4;DEFA5", "CTSS"))

FeaturePlot(scSeurat, features = c("FLT3", "S100A12"))

Look for B and plasma cell signatures

FeaturePlot(scSeurat, features = c("CD79B", "JCHAIN"))

Cluster 12 is the only cluster that is obviously dominated by the unstimulated experiments. What cells are these? Looks like they are hepatocytes. A lot of the markers are periportal, but all hepatocytes are in this cluster.

# Isolate DE genes
clust11 <- sCVdata_list$res.0.4@DEvsRest$`12`[order(sCVdata_list$res.0.4@DEvsRest$`12`$Wstat,
                                                    decreasing = TRUE), ]
rownames(clust11)[1:50]
 [1] "PAH"                                      "LOC114082496"                             "TDO2"                                     "MAT1A"                                    "GYS2"                                    
 [6] "SLCO1B3;SLCO1B3-SLCO1B7;SLCO1B7;SLCO1B1"  "FBP1"                                     "CPS1"                                     "SLC2A2"                                   "ALDOB"                                   
[11] "MTSS1"                                    "IGFBP1"                                   "CYP3A4;CYP3A7-CYP3A51P;CYP3A7-2"          "mikado.WCK01-AAH20201022-F8-rsc00143G668" "PCK1"                                    
[16] "SHMT1"                                    "CYP3A4;CYP3A7-CYP3A51P;CYP3A7"            "ARG1"                                     "CYP3A4;CYP3A7-CYP3A51P;CYP3A7-3"          "LOC107139912"                            
[21] "ALB-1"                                    "SLC38A4"                                  "LOC107139914"                             "SORBS2"                                   "TAT"                                     
[26] "C5-1"                                     "KLKB1"                                    "Slc22a19-1"                               "G6PC1"                                    "RBP4"                                    
[31] "AGMO"                                     "HSD17B11"                                 "CMBL"                                     "SLC25A13"                                 "ZBTB16"                                  
[36] "C8G"                                      "PYGL"                                     "HSD11B1"                                  "mikado.WCK01-AAH20201022-F8-rsc00060G101" "CES3"                                    
[41] "MTARC2"                                   "IDO2"                                     "AQP9"                                     "ACACB"                                    "GOT1"                                    
[46] "CFI"                                      "NR1I2"                                    "ABCB11"                                   "SOX5"                                     "BHMT2"                                   
FeaturePlot(scSeurat, features = c("PAH","PCK1"))

FeaturePlot(scSeurat, features = c("CYP2E1","FETUB"))

FeaturePlot(scSeurat, features = c("ACACB","ELOVL6"))

FeaturePlot(scSeurat, features = c("POLR2D", "ALB-1"))

Let’s look at some of the key markers that Sonya sent: IL2, IL4, IL6, IL7, IL10, IL12A, TGFB

FeaturePlot(scSeurat, features = c("IL2", "IL4"))

FeaturePlot(scSeurat, features = c("IL6", "IL6-1"))

FeaturePlot(scSeurat, features = c("IL7", "IL10"))

FeaturePlot(scSeurat, features = c("IL12A", "TGFB1"))

It’s pretty hard to see which cytokines are expressed in which samples, so I’m going to try splitting up the samples.

DimPlot(scSeurat, split.by = "tissue_type", label = TRUE) & NoLegend()
pdf(file = './figures/stimulation/downsampled_UMAP_split.pdf',
    height = 4, width = 8)
DimPlot(scSeurat, split.by = "tissue_type", label = TRUE) & NoLegend()
dev.off()
png 
  2 

Now let’s take a look at the features on this split plot.

genes <- c("IL2","IL4","IL6","IL7","IL10","IL12A","TGFB1","TGFB2","TGFB3","TNF","IL18","IL2RA","CCL5","IFNG","LIF", "CCL3L1;CCL3L3;CCL3;CCL18","CCL4L2;CCL4L1;CCL4")
for (gene in genes) {
  print(FeaturePlot(scSeurat, feature = gene, split.by = "tissue_type"))
  pdf(paste("figures/stimulation/downsampled_UMAP_split_", gene, ".pdf", sep = ""),
      height = 4, width = 8)
  print(FeaturePlot(scSeurat, feature = gene, split.by = "tissue_type"))
  dev.off()
}
Warning: All cells have the same value (0) of “IL2”

More plots

Cluster 2 looks like it generally has more IL genes expressed. Isolate DE genes for this cluster.

clust1 <- sCVdata_list$res.0.4@DEvsRest$`2`[order(sCVdata_list$res.0.4@DEvsRest$`2`$Wstat,
                                                    decreasing = TRUE), ]
cat(rownames(clust1)[1:50])
PTPRC TOX PRKCH INPP4B mikado.WCK01-AAH20201022-F8-rsc00190G443 ARID5B IKZF3 ARHGAP15 SATB1 DOCK10 TRPS1 RASA2 SAMSN1 NSD3 MBNL1 SH3KBP1 EMB SYTL3 ICOS mikado.WCK01-AAH20201022-F8-rsc00083G981 mikado.WCK01-AAH20201022-F8-rsc00275G164 THEMIS MSI2 SLAMF1 PITPNC1 NXF2;NXF2B;NXF5 SRSF7 IKZF1 RAPGEF6 STAT4 SLC38A1 RAP1A RABGAP1L ZC3HAV1 BCL2 TESPA1 APBB1IP GIMAP7 DHX8 DDX5 UBAC2 HUWE1 CD3E CTLA4 CD44 GIMAP1 RGS1 MTHFD1L RIPOR2 TANK

Visualise these genes in violin plots.

Interpretation: Now I see that IL2 and IL4 are expressed in more than just cluster 2, but it is nearly always in PMAIO. Cluster 8 is definitely where IL6 is expressed. IL7 is a bit more spread out across treatment types, but it’s definitely present in PMAIO in clusters 4, 5, and 11. Cluster 11 is plasma cells, but I’m honestly not sure of 4 and 5 - low signal hepatocytes? IL10 is in clusters 2 and 11 PMAIO. IL12A is a bit more in 4 and 5 PMAIO.

VlnPlot(scSeurat, feature = "IL2", split.by = "tissue_type") # Only in PMAIO cluster 2

VlnPlot(scSeurat, feature = "IL4", split.by = "tissue_type") # Only in PMAIO cluster 2

VlnPlot(scSeurat, feature = "IL6", split.by = "tissue_type") # Stronger in endo and mesem PMAIO

VlnPlot(scSeurat, feature = "IL6-1", split.by = "tissue_type") # No signal

VlnPlot(scSeurat, feature = "IL7", split.by = "tissue_type") # More in PMAIO cluster 4/5

VlnPlot(scSeurat, feature = "IL10", split.by = "tissue_type") # More in PMAIO cluster 2

VlnPlot(scSeurat, feature = "IL12A", split.by = "tissue_type") # A bit more in 1,4,5

VlnPlot(scSeurat, feature = "TGFB1", split.by = "tissue_type") # Pretty even across all

VlnPlot(scSeurat, feature = "TNF", split.by = "tissue_type")

Make Violin plots with ggplot

genes <- c("IL2","IL4","IL6","IL7","IL10","IL12A","TGFB1","TGFB2","TGFB3","TNF","IL18","IL2RA","CCL5","IFNG","LIF","CCL3L1;CCL3L3;CCL3;CCL18","CCL4L2;CCL4L1;CCL4")
for(gene in genes) {
  vln_df = data.frame(gene = scSeurat[["RNA"]]@data[gene,], 
                      Cluster = Idents(scSeurat), 
                      tissue_type = scSeurat$tissue_type)
  plot <- ggplot(vln_df, aes(x = Cluster, y = gene)) + 
    geom_violin(aes(fill = tissue_type), trim=TRUE, scale = "width") +
    ylab(gene) +
    theme_bw()
  print(plot)
  pdf(file = paste("figures/stimulation/downsampled_vlnPlot_split_", gene, ".pdf", sep = ""))
  print(plot) # Need print function because R doesn't autoprint in loops
  dev.off()
}

Looking for more genes

gene <- "IL18"
vln_df = data.frame(gene = scSeurat[["RNA"]]@data[gene,], 
                      Cluster = Idents(scSeurat), 
                      tissue_type = scSeurat$tissue_type)
  plot <- ggplot(vln_df, aes(x = Cluster, y = gene)) + 
    geom_violin(aes(fill = tissue_type), trim=TRUE, scale = "width") +
    ylab(gene) +
    theme_bw()
  print(plot)

Make heatmap with select genes

genes <- c("IL2","IL4","IL6","IL7","IL10","IL12A","TGFB1","TGFB2","TGFB3","TNF","IL18","IL2RA","CCL5","IFNG","LIF","CCL3L1;CCL3L3;CCL3;CCL18","CCL4L2;CCL4L1;CCL4")
DoHeatmap(scSeurat, features = genes, group.by = "integrated_snn_res.0.4",
          size = 3, 
          angle = 90, assay = "RNA", slot = "data") +
  NoLegend() +
  theme(text = element_text(size = 7))

DotPlot(scSeurat,
        assay = "RNA",
        features = genes,
        group.by = "integrated_snn_res.0.4",
        split.by =  "tissue_type")

DotPlot(scSeurat,
        assay = "RNA",
        features = genes,
        group.by = "tissue_type")
Warning: Scaling data with a low number of groups may produce misleading results

DotPlot(scSeurat,
        assay = "RNA",
        features = genes,
        group.by = "integrated_snn_res.0.4")

Perform DE test on T-cells from PMAIO vs unstim T-cells. I see TNF, IL2RA, but I also see hepatocyte genes like ALB-1, STAT3

cat(row.names(de_results)[1:100], sep = " ")
LOC107134468 CCL4L2;CCL4L1;CCL4 mikado.WCK01-AAH20201022-F8-rsc00073G191 MTHFD1L NFKB1 NXF2;NXF2B;NXF5 CCND3 IFNG SATB1 SKAP1 ARID5B mikado.WCK01-AAH20201022-F8-rsc00334G1 CCL3L1;CCL3L3;CCL3;CCL18 SLAMF1 CD200 GIMAP7 LOC114086011-6 GBP7;GBP4 CBLB TRAF3IP3 NR4A3 ALB-1 TNFRSF9 SLC25A26 SYNE1 STAT3 UTRN HUWE1 PDE7A IL2RA PTPRCAP APOA1 TNRC6B Ifi214;Ifi209 ETS1 ANKRD44 MAPK6 PDE3B RESF1 CD27 mikado.WCK01-AAH20201022-F8-rsc00138G153 PICALM JARID2 MDN1 CHIC1 CCL3L1;CCL3L3;CCL3;CCL18-2 CKLF;CKLF-CMTM1 HECA FOXN3 MED13L SEC23B ZEB2 AMN1 CD3G SEC14L1 BATF LNPEP IRF4 ALB LOC107134769-1 IQGAP2 LOC114086011-12 SERPINA1 mikado.WCK01-AAH20201022-F8-rsc00234G885 TMEM156 STK4 ATP8A1 PECAM1 FAM13A MTHFD2 CELF2 SAMSN1 NUCB2 GIMAP1 SND1 VMP1 MLLT3 ASAP1 RBP4 TNF CRTAM FYN ACTB mikado.WCK01-AAH20201022-F8-rsc00190G443 PPP3CC HBS1L TTC27 B4GALT5 DDX5 ARHGDIB LAT CXCL2;CXCL3;CXCL1-1 ITM2B SENP7 DOCK8 RIPOR2 APBB1IP TRAF3 SAMHD1 CD99
---
title: "Stimulation experiments"
output:
  html_document:
    df_print: paged
  html_notebook: default
  pdf_document: default
---

Analysis of stimulation experiments.

```{r}
library(Seurat)
library(dplyr)
library(ggplot2)
library(scClustViz)
library(SeuratWrappers)
```

Load integrated object. This object has been integrated with CCA, because there was lots of batch effect between the 0hr tissue and the other two experiments (which merged quite well).

```{r}
load("~/Dropbox/Zoe/scf_version/analysis/healthy_sc/seurat_objects/no_dropletQC/3391/integrated_3391_stimulation_pmaio_unstim_downsampled_cca_kanchor5_scClustViz.RData")
# Set identity
Idents(scSeurat) <- "integrated_snn_res.0.4"
```

Check out the UMAP of clusters, original identities, and refined SCINA labels.

```{r}
DimPlot(scSeurat, label = TRUE)
DimPlot(scSeurat, group.by = "tissue_type", label = FALSE)
DimPlot(scSeurat, group.by = "scina_labels", label = TRUE) & NoLegend()
```

```{r}
pdf(file = './figures/stimulation/downsampled_UMAP.pdf')
DimPlot(scSeurat, label = TRUE)
dev.off()
pdf(file = './figures/stimulation/downsampled_tissue_type_UMAP.pdf')
DimPlot(scSeurat, group.by = "tissue_type") +
  ggtitle(NULL)
dev.off()
```

Create UMAP of general cell-type labels

```{r}
plot <- DimPlot(scSeurat, group.by = "general_cell_labels_clusts", label = TRUE, repel = TRUE) +
  labs(title = NULL) & NoLegend()
plot
pdf("./figures/stimulation/downsampled_general_cell_type_UMAP.pdf",
    height = 5, width = 7)
plot
dev.off()
```


Look at tissue-type per cluster on a proportional level:

```{r}
pt <- table(scSeurat$general_cell_labels_clusts, scSeurat$tissue_type)
pt <- as.data.frame(pt)
plot <- ggplot(pt, aes(x = Var1, y = Freq, fill = Var2)) +
  theme_bw(base_size = 15) +
  geom_col(position = "fill", width = 0.5) +
  xlab("Cluster") +
  ylab("Proportion") +
  theme(legend.title = element_blank(),
        legend.position = "top")
#print(plot, vp = viewport(angle = 90))
plot + coord_flip()
pdf(file = './figures/stimulation/downsampled_tissue_type_barplot.pdf',
    height = 5, width = 7)
plot + coord_flip()
dev.off()
```

Calculate all DE genes

```{r}
all.markers <- FindAllMarkers(scSeurat,
                              only.pos = TRUE,
                              min.pct = 0.25, 
                              logfc.threshold = 0.25,
                              recorrect_umi = FALSE)
top.markers <- all.markers %>% group_by(cluster) %>% top_n(n = 10, wt = avg_log2FC)
top.markers
write.table(all.markers,
            file = "./figures/stimulation/downsampled_cluster_markers.tsv",
            sep = "\t",
            quote = FALSE,
            row.names = FALSE)
```

Calculate DE genes: for each cluster, compare stim VS unstim

```{r}
for (clust in levels(as.factor(Idents(scSeurat)))) {
  cells_stim <- colnames(scSeurat)[Idents(scSeurat) == clust & scSeurat$tissue_type == "6hrs_PMAIO"]
  cells_unstim <- colnames(scSeurat)[Idents(scSeurat) == clust & scSeurat$tissue_type == "6hrs_unstim"]
  markers <- RunPresto(scSeurat,
                       ident.1 = cells_stim,
                       ident.2 = cells_unstim,
                       only.pos = FALSE,
                       min.pct = 0.25,
                       logfc.threshold = 0.25,
                       recorrect_umi = FALSE)
  write.table(markers,
              file = paste("./figures/stimulation/downsampled_markers_cluster", 
                           clust ,"_stim_vs_unstim.tsv", sep = ""),
              sep = "\t",
              quote = FALSE,
              row.names = TRUE)
}
```

Look for T-cell signatures.

```{r}
FeaturePlot(scSeurat, features = c("CD3E", "NKG7"))
FeaturePlot(scSeurat, features = c("LEF1", "XCL1;XCL2"))
FeaturePlot(scSeurat, features = c("TOX", "CD8A"))
FeaturePlot(scSeurat, features = c("CCR7"))
```

Look for macrophage signatures:

```{r}
FeaturePlot(scSeurat, features = c("LGALS1", "TYROBP"))
FeaturePlot(scSeurat, features = c("MARCO", "C1QC"))
FeaturePlot(scSeurat, features = c("LYZ-1", "S100A8"))
FeaturePlot(scSeurat, features = c("DEFA6;DEFA4;DEFA5", "CTSS"))
FeaturePlot(scSeurat, features = c("FLT3", "S100A12"))
```

Look for B and plasma cell signatures

```{r}
FeaturePlot(scSeurat, features = c("CD79B", "JCHAIN"))
```


Cluster 12 is the only cluster that is obviously dominated by the unstimulated experiments. What cells are these? Looks like they are hepatocytes. A lot of the markers are periportal, but all hepatocytes are in this cluster.

```{r}
# Isolate DE genes
clust11 <- sCVdata_list$res.0.4@DEvsRest$`12`[order(sCVdata_list$res.0.4@DEvsRest$`12`$Wstat,
                                                    decreasing = TRUE), ]
rownames(clust11)[1:50]
FeaturePlot(scSeurat, features = c("PAH","PCK1"))
FeaturePlot(scSeurat, features = c("CYP2E1","FETUB"))
FeaturePlot(scSeurat, features = c("ACACB","ELOVL6"))
FeaturePlot(scSeurat, features = c("POLR2D", "ALB-1"))
```

Let's look at some of the key markers that Sonya sent: IL2, IL4, IL6, IL7, IL10, IL12A, TGFB

```{r}
FeaturePlot(scSeurat, features = c("IL2", "IL4"))
FeaturePlot(scSeurat, features = c("IL6", "IL6-1"))
FeaturePlot(scSeurat, features = c("IL7", "IL10"))
FeaturePlot(scSeurat, features = c("IL12A", "TGFB1"))
```

It's pretty hard to see which cytokines are expressed in which samples, so I'm going to try splitting up the samples.

```{r}
DimPlot(scSeurat, split.by = "tissue_type", label = TRUE) & NoLegend()
pdf(file = './figures/stimulation/downsampled_UMAP_split.pdf',
    height = 4, width = 8)
DimPlot(scSeurat, split.by = "tissue_type", label = TRUE) & NoLegend()
dev.off()
```

Now let's take a look at the features on this split plot.

```{r}
genes <- c("IL2","IL4","IL6","IL7","IL10","IL12A","TGFB1","TGFB2","TGFB3","TNF","IL18","IL2RA","CCL5","IFNG","LIF", "CCL3L1;CCL3L3;CCL3;CCL18","CCL4L2;CCL4L1;CCL4")
for (gene in genes) {
  print(FeaturePlot(scSeurat, feature = gene, split.by = "tissue_type"))
  pdf(paste("figures/stimulation/downsampled_UMAP_split_", gene, ".pdf", sep = ""),
      height = 4, width = 8)
  print(FeaturePlot(scSeurat, feature = gene, split.by = "tissue_type"))
  dev.off()
}
```

More plots

```{r}
FeaturePlot(scSeurat, feature = "IL15", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL16", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL17B", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL6ST", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL31RA", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL7R", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL7R-1", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL17D", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL5RA", split.by = "tissue_type") 
FeaturePlot(scSeurat, feature = "IL17RE", split.by = "tissue_type") 
FeaturePlot(scSeurat, feature = "IL17RC", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL17C", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL34", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL4I1", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL11", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL9R", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL4R", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL21R", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL27", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "ILK", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL18BP", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL10RA", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL3", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "CSF2", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IFNK", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL9", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL13", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "OSM", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "LIF", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "STAT1", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "STAT4", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL4", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL4I1", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "CD69", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "TLR7", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "HLA-DRA", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "IL12A", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "TLR2", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "NFKB1", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "ACTB", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "PTPRC", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "CD3E", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "TBP", split.by = "tissue_type")
FeaturePlot(scSeurat, feature = "SDHA", split.by = "tissue_type")
```


Cluster 2 looks like it generally has more IL genes expressed. Isolate DE genes for this cluster.

```{r}
clust1 <- sCVdata_list$res.0.4@DEvsRest$`2`[order(sCVdata_list$res.0.4@DEvsRest$`2`$Wstat,
                                                    decreasing = TRUE), ]
cat(rownames(clust1)[1:50])
```

Visualise these genes in violin plots.

**Interpretation**: Now I see that IL2 and IL4 are expressed in more than just cluster 2, but it is nearly always in PMAIO. Cluster 8 is definitely where IL6 is expressed. IL7 is a bit more spread out across treatment types, but it's definitely present in PMAIO in clusters 4, 5, and 11. Cluster 11 is plasma cells, but I'm honestly not sure of 4 and 5 - low signal hepatocytes? IL10 is in clusters 2 and 11 PMAIO. IL12A is a bit more in 4 and 5 PMAIO.

```{r}
VlnPlot(scSeurat, feature = "IL2", split.by = "tissue_type") # Only in PMAIO cluster 2
VlnPlot(scSeurat, feature = "IL4", split.by = "tissue_type") # Only in PMAIO cluster 2
VlnPlot(scSeurat, feature = "IL6", split.by = "tissue_type") # Stronger in endo and mesem PMAIO
VlnPlot(scSeurat, feature = "IL6-1", split.by = "tissue_type") # No signal
VlnPlot(scSeurat, feature = "IL7", split.by = "tissue_type") # More in PMAIO cluster 4/5
VlnPlot(scSeurat, feature = "IL10", split.by = "tissue_type") # More in PMAIO cluster 2
VlnPlot(scSeurat, feature = "IL12A", split.by = "tissue_type") # A bit more in 1,4,5
VlnPlot(scSeurat, feature = "TGFB1", split.by = "tissue_type") # Pretty even across all
VlnPlot(scSeurat, feature = "TNF", split.by = "tissue_type")
```

Make Violin plots with ggplot

```{r}
genes <- c("IL2","IL4","IL6","IL7","IL10","IL12A","TGFB1","TGFB2","TGFB3","TNF","IL18","IL2RA","CCL5","IFNG","LIF","CCL3L1;CCL3L3;CCL3;CCL18","CCL4L2;CCL4L1;CCL4")
for(gene in genes) {
  vln_df = data.frame(gene = scSeurat[["RNA"]]@data[gene,], 
                      Cluster = Idents(scSeurat), 
                      tissue_type = scSeurat$tissue_type)
  plot <- ggplot(vln_df, aes(x = Cluster, y = gene)) + 
    geom_violin(aes(fill = tissue_type), trim=TRUE, scale = "width") +
    ylab(gene) +
    theme_bw()
  print(plot)
  pdf(file = paste("figures/stimulation/downsampled_vlnPlot_split_", gene, ".pdf", sep = ""))
  print(plot) # Need print function because R doesn't autoprint in loops
  dev.off()
}
```

Looking for more genes

```{r}
gene <- "IL18"
vln_df = data.frame(gene = scSeurat[["RNA"]]@data[gene,], 
                    Cluster = Idents(scSeurat), 
                    tissue_type = scSeurat$tissue_type)
plot <- ggplot(vln_df, aes(x = Cluster, y = gene)) + 
  geom_violin(aes(fill = tissue_type), trim=TRUE, scale = "width") +
  ylab(gene) +
  theme_bw()
print(plot)
```

Make heatmap with select genes

```{r}
genes <- c("IL2","IL4","IL6","IL7","IL10","IL12A","TGFB1","TGFB2","TGFB3","TNF","IL18","IL2RA","CCL5","IFNG","LIF","CCL3L1;CCL3L3;CCL3;CCL18","CCL4L2;CCL4L1;CCL4")
DoHeatmap(scSeurat, features = genes, group.by = "integrated_snn_res.0.4",
          size = 3, 
          angle = 90, assay = "RNA", slot = "data") +
  NoLegend() +
  theme(text = element_text(size = 7))
DotPlot(scSeurat,
        assay = "RNA",
        features = genes,
        group.by = "integrated_snn_res.0.4",
        split.by =  "tissue_type")
DotPlot(scSeurat,
        assay = "RNA",
        features = genes,
        group.by = "tissue_type")
DotPlot(scSeurat,
        assay = "RNA",
        features = genes,
        group.by = "integrated_snn_res.0.4")
```


Perform DE test on T-cells from PMAIO vs unstim T-cells. I see *TNF, IL2RA*, but I also see hepatocyte genes like *ALB-1, STAT3*

```{r}
# Fetch cells to find out relevant cell barcodes
barcode_df <- FetchData(scSeurat, vars = c("tissue_type", "integrated_snn_res.0.4"))
barcode_df$barcode <- rownames(barcode_df)
# Filter on data subsets and pull barcode names
cells1 <- barcode_df %>%
  filter(tissue_type == "6hrs_PMAIO") %>%
  filter(integrated_snn_res.0.4 %in% c(2,6)) %>%
  pull(barcode)
cells2 <- barcode_df %>%
  filter(tissue_type == "6hrs_unstim") %>%
  filter(integrated_snn_res.0.4 %in% c(2,6)) %>%
  pull(barcode)
# Prep for DE testing
scSeurat <- PrepSCTFindMarkers(scSeurat)
# Perform DE test
de_results <- FindMarkers(scSeurat,
            ident.1 = cells1,
            ident.2 = cells2)
cat(row.names(de_results)[1:100], sep = " ")
```


